Transcriptional Activation bioassays, or reporter gene bioassays, are based in yeast cells or mammalian cells.
These bioassays can detect all compounds (structures) that are able to activate the androgen receptor and therefore have a hormonal action.
The assays are sensitive and specific, quick and simple to use and can be used to test biological samples such as plasma, serum and urine, supplement extracts, and other matrices such as feed.
The assays do not need to know the structure and therefore can identify unknowns in a sample.
The assays can be used as a first pass screen to identify which samples have androgenic activity. Chemical targeted analysis can be then used to identify the nature of the androgen in the sample.